Fig. 1: Cryo-EM structure of the stacked human DDB1-DDA1-DET1-COP1 complex.
From: Cryo-EM structure of the human COP1-DET1 ubiquitin ligase complex
A Cartoon schematic of the CRL4DET1-COP1 E3 ligase mediating the ubiquitination of c-Jun or ETS2. B Domain architecture of the DDB1-DDA1-DET1-E2-COP1 complex. BPA-BPC, β propeller A-C; CTD, C-terminal helical domain; UBC, ubiquitin-conjugating domain. C Coomassie blue-stained SDS-PAGE analysis of the purified DDB1-DDA1-DET1-E2-COP1 complex. The experiment was repeated at least three times independently with similar results. Source data are provided as a Source Data file. D Representative 2D class averages of the stacked human DDB1-DDA1-DET1-E2-COP1 complex. E The unsharpened cryo-EM map of the stacked DDB1-DDA1-DET1-E2-COP1 complex. F Refined coordinates of the stacked complex, visualized in two layers from different views. G The refined model of a single DDB1-DDA1-DET1-E2-COP1 complex associated with endogenous Ube2e. H Zoomed-in view of the interface between DET1, 2 copies of COP1 and Ube2e2. I Close-up of the diamond-shaped arrangement formed by coiled-coil domains among eight copies of COP1 molecules in the stacked DDB1-DDA1-DET1-E2-COP1 complex. CC, coiled-coil.
