Fig. 2: Architecture of the dimeric human DDB1-DDA1-DET1-E2-COP1 complex.
From: Cryo-EM structure of the human COP1-DET1 ubiquitin ligase complex
A DeepEMhancer post-processed cryo-EM density map (left) and atomic model (right) of the dimeric DDB1-DDA1-DET1-E2-COP1 complex. The subunits are colored as follows: DDB1, orange; DDA1, purple; DET1, thistle; COP1, dark sea green; Ube2e, cornflower blue. B Zoomed-in views of the interface showing DET1 binding to Ube2e and two copies of COP1. C Cartoon representation of DET1 highlighting interaction regions with different binding proteins. D, E Close-up view of the molecular interface between DET1 and COP1WD40a or COP1WD40b. The key residues contributing to the interaction are labelled. F Pull-down experiment of wild type or key binding residues mutants of TSF-DET1, GST-COP1 and His-Ube2e2, the eluents were analysed by western blotting. TSF, twin-strep-FLAG tag. Data in F are representative of three independent experiments. Source data are provided as a Source Data file.
