Fig. 5: Comparison of GenSLM-TrpB 230 and its closest natural homolog from Neobacillus drentensis (NdTrpB).
From: Sequence-based generative AI design of versatile tryptophan synthases
A Product yields with the native substrate at room temperature and 75 °C, as well as with various non-canonical substrates. Bar heights represent the mean of technical triplicates (n = 3). Source data are provided as a Source Data file. B Structural alignment predicted by AlphaFold355 (backbone RMSD = 0.36 Å); 230 is shown in orange, NdTrpB in green, and non-conserved residues are highlighted in purple. The close-up depicts the active site residues within 5 Å of PLP. Only one active site residue differs between the two structures; the mutation from 230 to NdTrpB is indicated.
