Fig. 2: Correlation of TIMP-1, COL1A1, ROS and AQP5. | Nature Communications

Fig. 2: Correlation of TIMP-1, COL1A1, ROS and AQP5.

From: A nanosystem targeting tissue inhibitor of metalloproteinase-1 for continuous spatiotemporal idiopathic pulmonary fibrosis therapy

Fig. 2: Correlation of TIMP-1, COL1A1, ROS and AQP5.

a Schematic illustration of the experimental process. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/24749x6). b IHC staining and quantification of TIMP-1 and COL1A1 in lung sections from non-IPF controls (n = 10 non-IPF patients) and IPF patients (n = 8 IPF patients). c Correlation analysis of TIMP-1 and COL1A1. d Schematic illustration of the experimental process. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/chpxtxr). e IHC staining and quantification of TIMP-1 and COL1A1 in lung sections from controls and bleomycin-induced fibrosis mouse models. f Correlation analysis of TIMP-1 and COL1A1 (n = 20 samples). Immunofluorescence (IF) staining of ROS, IHC staining of AQP5, and the quantification of positive area in lung sections from non-IPF controls (n = 5 non-IPF patients for ROS; n = 10 non-IPF patients for AQP5) and IPF patients (n = 10 IPF patients for ROS; n = 8 IPF patients for AQP5) (g), as well as bleomycin-induced fibrosis mouse models (n = 10 mice) (h). Data indicate mean ± SEM, two-tailed unpaired Student’s t test. Source data are provided as a Source Data file.

Back to article page