Fig. 3: Homology analysis of BREX type 4 systems and evidence of PT synthesis.
From: Phosphorothioate DNA modification by BREX type 4 systems in the human gut microbiome
A The genomic organization of the BREX type 4 gene systems in Bacteroides salyersiae and putative Butyricimonas faecalis. Vibrio cyclitrophicus FF75 is included to demonstrate a typical ssp gene system and Escherichia coli HS to demonstrate a typical BREX type 1 system. Protein domains are color-coded and labeled. B The levels of PT dinucleotides in engineered B. salyersiae and Bacteroides thetaiotaomicron strains. ΔbrxC, ΔbrxC brxCBf, ΔbrxC sspCBo, vector, and brxCBs all differ significantly from wild-type (WT) based on a two-tailed Student’s t-test with three biological replicates (p < 0.05). Data are presented as mean values ± SD. Bs: B. salyersiae, Bf: Butyricimonas faecalis, Bo: Bacteroides ovatus. Statistical data are provided in Supplementary Data 15 and 16.
