Fig. 2: TCR activity, RNAPII clearance and RNAPII degradation in an isogenic collection of TCR knockout cells.

a Representative images of recovery of RNA synthesis (RRS), measured by incorporation of 5-EU (1 h labeling), either mock-treated (-UV) or 3 h or 24 h after UV-C irradiation (9 J/m²). Only WT and CSB^KO are shown here. See Supplementary Fig. 2a for the other conditions. b Quantification of RRS as in (a). The nascent transcription levels of the indicated cell lines, where 5-EU levels were normalized to the mock treatment for each cell line. The experiment was performed three times. Each transparent colored circle represents one cell. Each dark circle represents the mean of two technical replicates, with more than 50 cells collected per technical replicate. The black lines represent the mean of all three independent experiments. c Representative images of the local DRB run-off assay, as in Fig. 1a-b in WT, CSB^KO, and UVSSA^KO cells. See Supplementary Fig. 3a for representative images of the other conditions. Scale bar, 10 μm. d Quantification of (c) for the indicated RPE1 WT and TCR^KO cells as described for Fig. 1c. e A representative image of the global DRB run-off assay by western blotting in the indicated RPE1 WT and TCR^KO cells as described for Fig. 1e. The dashed line indicates the merge zone of cropped blots. f Quantification of (e) as described for Fig. 1f-g.