Fig. 5: Kla reduction recovers abnormal uterine receptivity in PCOS mice.
a Schematic diagram of PCOS mice construction and drug intervention. Created in BioRender. Hongying, S. (2025) https://BioRender.com/nwdxi9x. b ISs visualized by blue dye in naturally conceived mice. Black arrows indicate ISs. c The number of ISs in naturally conceived mice on day 5 (Oil, n = 7; DHEA, n = 6; DHEA + 0.3 mg/kg Oxamate, n = 6; DHEA + 1.5 mg/kg Oxamate, n = 6; DHEA + 2.25 mg/kg Oxamate, n = 7; DHEA + 3 mg/kg Oxamate, n = 5; biologically independent mice). d Percentage of naturally conceived mice with ISs on day 5. e, f H3K18la levels in the murine uterus on day 4 (n = 5, biologically independent mice). g H3K18la IF staining in the murine uterus on day 4. h H&E, Ki67 IHC, and MUC1 IF staining in the murine uterus on day 4. i, j Protein levels of ERα in the murine uterus on day 4 (n = 8 for Oil, n = 7 for DHEA and DHEA + Oxamate, biologically independent mice). k Relative mRNA levels of Esr1, Msx1, Msx2, and Ltf in the murine uterus on day 4 (n = 6, biologically independent mice). Exact P values: c Oil vs. DHEA, P = 2.33 × 10−4; f Oil vs. DHEA, P = 4.23 × 10−4; k For relative mRNA levels of Esr1, Oil vs. DHEA, P = 7.84 × 10−14, DHEA vs. DHEA + Oxamate, P = 2.99 × 10−8; for relative mRNA levels of Msx2, Oil vs. DHEA, P = 9.85 × 10−5, DHEA vs. DHEA + Oxamate, P = 6.99 × 10−5; for relative mRNA levels of Ltf, Oil vs. DHEA, P = 9.89 × 10−5. For f and relative mRNA levels of Esr1, Msx1, and Msx2 in (k), P values were determined by one-way ANOVA followed by Tukey’s post hoc test, and data are presented as means ± s.e.m. For c, j and relative mRNA levels of Ltf in (k), P values were determined using Kruskal–Wallis test followed by Dunn’s post hoc test, and data are presented as medians with interquartile ranges. For g, h, images are representative of four independent biological replicates. Scale bar: 100 μm. Source data are provided as a Source data file.
