Fig. 2: Overview of microbial diversity and changes associated with FMT. | Nature Communications

Fig. 2: Overview of microbial diversity and changes associated with FMT.

From: Impact of a single fecal microbiome transplantation in adult women with anorexia nervosa: an open-label feasibility pilot trial

Fig. 2: Overview of microbial diversity and changes associated with FMT.The alternative text for this image may have been generated using AI.

A–F Overview of microbial diversity and compositional changes associated with FMT. A, B Alpha diversity of donor samples and participant microbiota pre- (w0) and post (w1)-FMT measured using Chao1 richness (A) and Shannon evenness (B) indices, stratified by donor source (Don1 = blue (n = 3), Don2 = green (n = 10)) using 1 biological sample for each. Donor versus w0 is tested by one-sample t-test towards the mean of the donor, while change from w0 to w1 is by mixed linear model with patient and time as factors. Both tests are two-sided. Significant changes are indicated (*p < 0.05, ***p < 0.001). Actual p values are: 2a: p(Donor1 vs w0) = 0.020, p(Donor1, w0 vs w1) = 0.153, p(Donor2 vs w0) = 0.020, p(Donor2, w0 vs w1) = 0.0317. 2b: p(Donor1 vs w0) = 0.058, p(Donor1, w0 vs w1) = 0.602, p(Donor2 vs w0) = 0.010, p(Donor2, w0 vs w1) = 0.0500, 2d: p = 4.1e-04. C Beta diversity visualized by dbRDA plot, showing Bray-Curtis dissimilarities of microbial communities. Points are colored by donor and timepoint (Purple=w0 and dark blue (w1_don1) or dark green (w1_don2)=w1), illustrating shifts (n = 18) toward donor profiles after FMT. D Distance to donor microbiota pre- (w0) and post (w1)-FMT, stratified by donor (Don1 = blue, Don2 = green). We used a linear mixed model with patient as random effect, and time as systematic factor. A significant reduction in distance post-FMT is observed (***p < 0.001). E Volcano plots of differentially abundant taxa between donor and participant samples, highlighting key families associated with each donor (e.g., Lachnospiraceae and Bacteroidaceae for Don1, Christensenellaceae and Eggerthellaceae for Don2). Vulcano-plot based on DEseq2 parametric differential abundance testing with wald-test for specific contrasts. F Differentially abundant microbial pathways post-FMT, showing changes in pathway categories across donor groups based on log2 fold change. Boxplots of log-fold change as function of inference (p value categories). Box from 25–75 percentile (IQR), line in box is median, whiskers are from min to max, and points reflect outliers defined as 1.5*IQR above (below) 75 (25) percentiles.

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