Fig. 2: Hearts of Wnt1-Cre; Nedd4fl/fl embryos exhibit shortened outflow tract and incomplete outflow tract rotation. | Nature Communications

Fig. 2: Hearts of Wnt1-Cre; Nedd4fl/fl embryos exhibit shortened outflow tract and incomplete outflow tract rotation.

From: Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development

Fig. 2: Hearts of Wnt1-Cre; Nedd4fl/fl embryos exhibit shortened outflow tract and incomplete outflow tract rotation.

A Hearts of wildtype and Wnt1-Cre; Nedd4fl/fl E10.5 embryos showing expression of Mef2cAHF-LacZ reporter gene in the right ventricle (RV) and outflow tract (OFT). Right panel: OFT + RV length was measured as the distance along the dashed lines as indicated. LV left ventricle. Data points represent biological replicates from n = 7 wildtype and n = 5 Wnt1-Cre; Nedd4fl/fl embryos from three independent experiments. Graph represents mean +/− SEM, ****P = 0.000011 (unpaired t test, two-tailed). B Coronal sections through the outflow tract region of wildtype and Wnt1-Cre; Nedd4fl/fl E12.5 embryos immunostained for smooth muscle actin (SMA) and CD31. Representative images from n = 6 embryos per genotype from six independent experiments. Incomplete clockwise outflow tract rotation is observed in Wnt1-Cre; Nedd4fl/fl embryos, evidenced by misalignment of the aortic valve over the right ventricle. Valve leaflet defects are also observed. L left, R right, A aortic artery/valve, P pulmonary artery/valve. Source data are provided as a Source Data file.

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