Fig. 1: In vivo HSPC CRISPR screen identifies SAGA complex members as putative regulators of hematopoiesis.
From: In vivo CRISPR screening identifies SAGA complex members as key regulators of hematopoiesis
a Schematic of the in vivo genome-wide (GW) CRISPR knockout (KO) screen for hematopoiesis. b Volcano plot showing in vivo hematopoietic stem and progenitor cell (HSPC) CRISPR GW screen results comparing HSPC KOs with KO mature lineage cells. All KOs displayed with the effect of KO (negative if KO enriched in HSPCs, positive if KO enriched in mature lineage cells) on the x-axis and confidence score on the y-axis. SAGA complex members labeled in red. c Gene Ontology (GO) enrichment of top 200 screen hits ranked by confidence score. GO term enrichment p-values were computed using Fisher’s exact test. Reported adjusted p-values correspond to Benjamini–Hochberg FDR correction. d Volcano plot showing in vivo HSPC CRISPR screen results for sub-library of 2000 genes involved in gene expression. All KOs displayed with the effect of KO (negative if KO enriched in HSPCs, positive if KO enriched in differentiated cells) on the x-axis and confidence score is on the y-axis. SAGA complex members labeled in red. Volcano plot showing in vivo HSPC sub-library CRISPR screen results comparing c-Kit+Lineage- HSPC KOs with KO mature T-cells (e), mature B-cells (f), myeloid cells (g), and erythroid cells (h). All ~2000 gene KOs displayed with the effect of KO (negative if KO enriched in HSPCs, positive if KO enriched in mature lineage cells) on the x-axis and confidence score on the y-axis. SAGA complex members labeled in red.
