Fig. 3: Virions form and mature in the immediate vicinity of replication organelles.

A Slice through tomogram of LGTV-infected cell showing an immature virion budding across from a RO (orange arrow), and mature virions (red arrows) in adjacent membranes. B Segmentation of the tomogram in (A), with color labels defined for each structure. C Slice through tomogram of LGTV-infected showing mature (red arrows) and immature (orange arrows) LGTV virions observed near the viral RO. D, E Subtomogram averages of immature (D) and mature (E) LGTV from cellular tomograms. F The distance from immature (N = 37) and mature (N = 24) virions to the closest RO, from five tomograms. Bars indicate average±standard deviation. Statistical significance by unpaired two-tailed Student’s t test, p = 0.0003. G 3D-SIM maximum intensity projection of fixed, LGTV-infected cells at 24 h p.i., stained for the ER marker Calnexin, the Golgi marker GM130, and the viral RNA replication intermediate dsRNA. Colors as per inset. H–J Zoomed region from (G) showing each marker separately. K Merge of the three markers with axial views (x–z, bottom; x–y, right) corresponding to the dashed lines in (K). Scale bars 100 nm (A, C), 5 µm (G), 2.5 µm (H–J), 1 µm (K).