Fig. 6: Crystal structure of anti-Ripr mAb 5B3 in complex with PvRiprEGF7-8.
a Crystal structure of PvRiprEGF7-8 (yellow) in complex with 5B3 Fab, with heavy chain in dark blue and light chain in light blue. b Sequence alignment of the EGF 7-8 domains from PvRipr, PkRipr, PcRipr and PfRipr. Similarity was predicted using ESPript 3.0 with the “% equivalent” parameter scheme. Identical residues are shown in pink, residues with similar physicochemical properties are in gray and residues with distinct properties in cyan. Mutated N-linked glycosylation sites (Asn767Gln and Asn780Gln) are marked with asterisks. Below is the surface area contribution of each PvRipr EGF 7-8 residue buried by antibody 5B3 as determined by PISA. Residues forming hydrogen bonds of salt bridges with 5B3 are colored black; those involved in van der Waals interactions are gray. c Surface representation of the PvRiprEGF 7-8 with the 5B3 epitope colored according to sequence similarity as in b. d, e Detailed views of interactions between 5B3 and polymorphic Ripr residues Glu786 in d and Asn802 in e. Hydrogen bonds and salt bridges are shown as black dashed lines.
