Fig. 5: Single cell RNA-sequencing of in vivo bradyzoites.
From: Bradyzoite subtypes rule the crossroads of Toxoplasma development
a UMAP projection of individual in vivo bradyzoites from 30-day infected CBA mice shows five potential subtypes; Groups A, B, C, D, and E with the number of bradyzoites analyzed indicated below each group label. b A Venn Diagram of significant gene expression (P < 0.05) from bradyzoite Groups A-E was generated. This analysis shows all common, partially shared and unique genes associated with each Group. c Table indicates the total number of genes in Groups A-E that are significantly expressed (Supplementary Data 1, P < 0.05) and the breakdown of shared expression with the published G1 and S/M sub-transcriptomes14,27,37,63 as well as the number of non-cell cycle genes. d Volcano plots for each Group A-E were generated in comparison to BAG1+ bradyzoites in day 7 ex vivo cultures of infected astrocytes. Grey indicates genes with p > 0.05, red indicated significantly upregulated genes (P < 0.05) and blue indicates downregulated genes (P < 0.05). Up to 15 upregulated and 5 downregulated genes are indicated on each volcano plot. Differential expression was assessed using Seurat’s Wilcoxon rank-sum test (FindMarkers), with Benjamini-Hochberg correction for multiple testing. Results are presented as volcano plots of log2 fold change versus -log10 adjusted p-value. Source data are provided as a Source Data file for this figure.
