Fig. 2: Micrograph of sealed sample after laser irradiation.
From: Ultrathin liquid cells for microsecond time-resolved cryo-EM
a An apoferritin cryo sample was flash melted with 10 laser pulses of 30 µs duration. The region in the center of the laser focus has been revitrified (green), while surrounding areas have not reached the melting point and have crystallized (purple). Areas even further from the center of the laser focus have remained vitreous (blue). Three holes are highlighted in yellow that crystallized after the first laser pulse but subsequently turned vitreous. b, c Details of the revitrified areas showing intact apoferritin particles. A diffraction pattern of the area in b (inset) confirms that the sample is vitreous. Scale bars, 2.5 µm in a, 100 nm in b, c, and 1 Å−1 in the inset of b. Note that the experiment was repeated 155 times with similar results for the data shown in Fig. 4c.
