Fig. 5: Lack of FUNDC1 downregulates GATA2 to inhibit ET-1 production. | Nature Communications

Fig. 5: Lack of FUNDC1 downregulates GATA2 to inhibit ET-1 production.

From: Endothelial FUNDC1 regulates metabolic reprogramming and the obesity-diabetes transition through the SIRT3/GATA2/endothelin-1 axis

Fig. 5: Lack of FUNDC1 downregulates GATA2 to inhibit ET-1 production.

a HUVECs were transfected with control siRNA (siCtrl) or FUNDC1 siRNA (siFUNDC1) and subjected to immunoblotting analysis. b Quantification of protein levels of ECE1, GATA2, and AP1. n = 6 independent experiments. c HUVECs were transfected with control siRNA (siCtrl) or FUNDC1 siRNA (siFUNDC1) and subjected to Q-PCR analysis to detect ECE1, AP1, and JUN mRNA levels. n = 8 independent experiments. d HUVECs were transfected with control Myc-Flag-tagged plasmid (Myc-Flag-Ctrl) or Myc-Flag-FUNDC1 plasmid (Myc-Flag-FUNDC1) and subjected to immunoblotting analysis. e Quantification of protein levels of FUNDC1, ECE1, GATA2 and AP1 in (d). n = 6 independent experiments. f HUVECs were transfected with control siRNA (siCtrl) or GATA2 siRNA (siGATA2) and subjected to Q-PCR analysis to detect EDN1 mRNA levels. n = 8 independent experiments. g HUVECs were transfected with control siRNA (siCtrl) or FUNDC1 siRNA (siFUNDC1) and were then treat with 200 µM PA for indicated time periods. h-j Quantification of protein levels of FUNDC1, GATA2, and ET-1 in (g). n = 4 independent experiments. Data are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. p > 0.05.

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