Fig. 3: Endovascular injury-activated MSCs exhibit heightened immunoregulatory features. | Nature Communications

Fig. 3: Endovascular injury-activated MSCs exhibit heightened immunoregulatory features.

From: Perivascular mesenchymal cells instruct ST2+ reparative macrophages to promote endovascular injury-induced neointimal hyperplasia in mice

Fig. 3: Endovascular injury-activated MSCs exhibit heightened immunoregulatory features.The alternative text for this image may have been generated using AI.

a–i Analysis of the scRNAseq datasets of D0, D2 and D7 groups presented in Fig. 1. a Chord diagram visualization of the frequency of interactions between the 11 cell populations. b Total numbers of the identified ligand-receptor interactions between the 11 cell populations. c Heat map showing the ligand-receptor interaction numbers between each pair of the 11 cell populations using CellChat. d UMAP visualization of the MSC subpopulations (MSC1 and MSC2). e Pseudotime analysis clarifying the developmental trajectory of the MSCs. f UMAP visualization of individual MSC subsets in the indicated groups. g Signaling pathways enriched in MSC2 cluster using GO_BP. h UMAP plot showing Pdpn gene expression in MSCs. i Violin plot showing the expression of Pdpn gene in the 2 MSC subsets. j Representative images of the flow cytometry analysis of PDPN expression in arterial PDGFRβ + cells at D0 and D7. k gMFI of PDPN in MSCs from the indicated groups. n = 6 mice per group. l Relative frequencies (%) of PDPN+ MSCs in the indicated groups. n = 5 mice per group. m PDGFRβ + PDPN + MSCs were retrieved from femoral arteries of 8-week-old male mice at D0 and D7 for bulk RNA sequencing (Bulk RNA-seq) analysis. Each sample was pooled cells from n = 5 mice. n PCA plot of the bulk RNA-seq datasets. o Signaling pathways enriched in the MSCs from the indicated groups, analyzed using GSEA and ranked by NES. Data are all shown as the mean ± s.e.m. ***p < 0.001 by unpaired two-tailed Student’s t-test (k,l). Source data and statistic information are provided as a Source data file. Figure 3a,c,m were created in BioRender. Shan, B. (2025) https://BioRender.com/x16x8wd.

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