Fig. 6: IL-33-activated arterial macrophages promote endovascular injury-induced VSMC proliferation and neointima formation. | Nature Communications

Fig. 6: IL-33-activated arterial macrophages promote endovascular injury-induced VSMC proliferation and neointima formation.

From: Perivascular mesenchymal cells instruct ST2+ reparative macrophages to promote endovascular injury-induced neointimal hyperplasia in mice

Fig. 6: IL-33-activated arterial macrophages promote endovascular injury-induced VSMC proliferation and neointima formation.The alternative text for this image may have been generated using AI.

a Arterial Il1rl1mRNA levels at D0 (n = 4), D4 (n = 7), D7 (n = 8) and D14 (n = 4). b Relative human IL1RL1 RNA abundance in the left internal mammary artery from individuals with stent implantation or controls (GSE19136). c Representative images of flow analysis of ST2 expression in CD45+ cells. d,e Cell frequencies (d) and counts (e) of ST2+ immune cells in the arteries at D0 (n = 3), D2 (n = 6), D7 (n = 6). f–j Flow analysis of arteries of Pb-Il33KO and control mice at D7. f, Representative images of the flow analysis of ST2 expression in arterial CD45+ cells. g,h Cell frequencies (g) and counts (h) of ST2+ immune cells in Control (n = 6) and Pb-Il33KO (n = 5) mice. i tSNE visualization of the integrated data of all ST2+ immune cells in the artery. 7 cell populations were annotated and color-coded. j, Cell counts of arterial ST2+ immune cell subsets. n = 8 mice each group. k (Left) Venn diagram showing the 451 common DEGs of the two indicated gene sets. IL33UP, IL-33-upregulated genes (GSE151653); D7_MacUP, D7_Mac upregulated genes. (Right) GSEA analysis of the 451 DEGs. l Representative images of the flow analysis of ST2 expression in total macrophages at D2, or in LY6C+ and LY6C- subsets at D7. m Representative images of the flow analysis of ST2+ cells within RepMACs at D7. n Il1rl1 mRNA levels in BMDMs from Control and Mac-Il1rl1KO mice. o,p Representative images of (o) H&E and (p) αSMA staining. Intima/Media ratios and lumen diameter/aspect ratios were quantified using the artery sections of Control (n = 6) and Mac-Il1rl1KO (n = 7) mice at D28. Scale bars, 50 μm. q,r Representative images (q) and cell counts (r) of Ki67-expressing VSMCs of Control (n = 6) and Mac-Il1rl1KO (n = 7) mice at D7 using flow analysis. s, Representative images of αSMA and EdU staining using artery sections at D7 (n = 6 mice). EdU+ VSMCs per HPF were quantified. Scale bars, 50 μm. Data are all shown as the mean ± s.e.m. *p < 0.05, **p < 0.01 or ***p < 0.001 by unpaired two-tailed Student’s t-test (b,g,h,j,n,o,r,s), or one-way ANOVA (a,d,e). Source data and statistic information are provided as Source data files.

Back to article page