Fig. 5: Classification and activity of hydrogenases. | Nature Communications

Fig. 5: Classification and activity of hydrogenases.

From: Reduced methane emissions in transgenic rice genotypes are associated with altered rhizosphere microbial hydrogen cycling

Fig. 5: Classification and activity of hydrogenases.

Phylogeny of identified [NiFe] hydrogenases (a) and [FeFe] hydrogenases (b). Black dots indicate support values ≥ 80% calculated based on 1000 replicates. Dashed branches are references. c Transcriptional activity of classified hydrogenase groups in different rice types over the cultivation period. d Transcriptomic abundances of [NiFe] Group 1 hydrogenases resolved by organisms of origin, and the combination of [FeFe] Group A1 and [NiFe] Group 4. Error bars indicate standard deviations of sum values in replicates (n = 3 for bulk soil and n = 5 for rice genotypes). Symbols (i.e., circles, triangles, diamonds) indicate significant differences between wild type (Kitaake) and PSY genotypes (PSY1-17 and PSY1-9) at the same cultivation time, tested by the Wilcoxon Rank Sum Test. For [NiFe] Group 1 hydrogenases in Kitaake versus PSY1-17, p = 0.030 (two-sided) on 15 days and p = 0.036 (two-sided) on 70 days; in Kitaake versus PSY1-9, p = 0.037 (two-sided) on 70 days. For the combination of [FeFe] Group A1 and [NiFe] Group 4, p = 0.012 (two-sided) in Kitaake versus PSY1-17 and p = 0.020 (two-sided) in Kitaake versus PSY1-9 on 30 days.

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