Fig. 2: Validation of uterine decellularization and characterization of the resulting ECM scaffolds. | Nature Communications

Fig. 2: Validation of uterine decellularization and characterization of the resulting ECM scaffolds.

From: Decidualization-empowered ECM hydrogel integrating sustained Tβ4 release drives endometrial regeneration in intrauterine adhesions

Fig. 2: Validation of uterine decellularization and characterization of the resulting ECM scaffolds.The alternative text for this image may have been generated using AI.

Uterine tissues from control (Con) and decidualized (Dec) horns were analysed before (Pre) and after the decellularization process. a Macroscopic images. b Haematoxylin and eosin (H&E) staining. c Masson’s trichrome staining. d Quantification of collagen fibre area based on images in (c). e Quantification of residual DNA content. f–h Representative immunofluorescence images and quantification of mean fluorescence intensity (MFI) for Collagen I (COL1, green) (f), Collagen IV (COL4, red) (g), and Fibronectin (FN1, red) (h). Nuclei are counterstained with DAPI (blue). Scale bars, 100 µm (b, c); 50 µm (f–h). Images in (a–c) are representative of (n = 6) biologically independent samples with similar results. Data are presented as mean ± s.d. (n = 6 (d), n = 10 (e), n = 3 (f–h) biologically independent samples). Statistical significance was assessed using an unpaired two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Detailed statistical analyses, including exact P values, test statistics (F values and degrees of freedom), and 95% confidence intervals, are provided in the Source Data file.

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