Fig. 1: RUVBL1 associates co-translationally with mRNAs.
From: Co-translational determination of quaternary structures in chaperone factories
A Schematic of the HSP90/R2TP chaperone. DII is the domain II of RUVBL1 and RUVBL2. Created in BioRender, Tartier, S. (2026) https://BioRender.com/5ulccbz.B Schematic of the cassette integrated at the RUVBL1 genomic locus and micrographs of HCT116 cells with a RUVBL1-GFP allele and imaged by fluorescent microscopy. Green: RUVBL1-GFP. Blue: DAPI staining. Scale bar: 5 µm. C Volcano plot of the RUVBL1-GFP RIP-seq experiment, showing the pValue (y axis; -Log10) as a function of the enrichment in the GFP IP over the control IP (x axis; Log2 (GFP IP/control IP)). Each dot represents an mRNA that is colored according to the code on the right of panel E. The statistical test was a two-sided QL F-test. D Volcano plot of the RUVBL1-GFP RIP-seq experiment in cells treated with puromycin for 1 h. Legend as in (C). E Volcano plot comparing the RUVBL1-GFP RIP-seq in untreated versus CB-6644-treated condition (16 h). The graph shows the p-value (y axis; -Log10) as a function of the fold change in the GFP IPs of treated versus non-treated cells (x axis; Log2(GFP IP/control IP)). The statistical test was a two-sided QL F-test.
