Fig. 6: Translation dependency of the colocalization of POLR2A and PRPF8 mRNAs with endogenous RPAP3. | Nature Communications

Fig. 6: Translation dependency of the colocalization of POLR2A and PRPF8 mRNAs with endogenous RPAP3.

From: Co-translational determination of quaternary structures in chaperone factories

Fig. 6: Translation dependency of the colocalization of POLR2A and PRPF8 mRNAs with endogenous RPAP3.The alternative text for this image may have been generated using AI.

A Micrographs of HeLa Kyoto cells treated or not with puromycin and labeled for the POLR2A mRNA by smFISH (left) and for the endogenous RPAP3 protein by immunofluorescence (middle). Merge images (right) show POLR2A mRNA in red, RPAP3 protein in green, and DAPI staining in blue. Scale bars: 10 µm. The inset shows a higher magnification of the boxed area. This experiment was repeated three times independently with similar results. B Micrographs of wild-type HeLa Kyoto cells treated or not with puromycin, and labeled for POLR2A (left) and PRPF8 (middle) mRNAs by smFISH. Merge images (right) show POLR2A mRNA in red, PRPF8 mRNA in green, and DAPI staining in blue. Scale bars: 10 µm. The inset shows a higher magnification of the boxed area. This experiment was repeated three times independently with similar results.

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