Table 1 Primers used for RT-qPCR
From: Co-translational determination of quaternary structures in chaperone factories
Targeted RNA | Forward Primer | Reverse Primer | Amplicon size |
|---|---|---|---|
POLR2A | 5’ CCCCAACCTCTCCATTGACC 3’ | 5’ CCCTGCGCACTAGTTCTTGA 3’ | 116 |
POLR2B | 5’ TTCTTCCCGACCCACCAGTA 3’ | 5’ GTAGAGTTGCCACAATGCGC 3’ | 101 |
POLR2C | 5’ GGATGAGGATGAGTCGCAGG 3’ | 5’ GGGCTGACAGGACAATGGTT 3’ | 113 |
POLR2H | 5’ CCGTCAGGGGTGAAAAGCAA 3’ | 5’ GGGAATTTGCCATGACGTCG 3’ | 107 |
EFTUD2 | 5’ CAGTCAACCCCTCAGAGCTG 3’ | 5’ ATCACATGCTCGCCAGACTC 3’ | 106 |
PRPF8 | 5’ TCACCACCCATGCCAAGATC 3’ | 5’ TAGGCCGTCAGTGTACAGGA 3’ | 106 |
EP400 | 5’ GGCTGCTCAGGGAAATGACT 3’ | 5’ AACTCCTCAGCTTTGACCGG 3’ | 117 |
BRD8 | 5’ AGCCAGTCATTTCAGGAGCC 3’ | 5’ CTCCAGCTGCAGTACTTCCC 3’ | 117 |
mCherry | 5’ AGTTGGACATCACCTCCCAC 3’ | 5’ CTTGTACAGCTCGTCCATGC 3’ | 101 |
