Fig. 3: Analysis of adipose and muscle obtained from mice bearing isogenic H1573 NSCLC tumors.
A–J NOD/SCID mice were treated as described in Fig. 2 (n = 6 per group). Mice were sacrificed with epididymal white adipose tissue (eWAT) (A), inguinal white adipose tissue (iWAT) (B), gastrocnemius muscle (F), and quadriceps muscle (G) weighed. eWAT and gastrocnemius muscle were harvested and processed for H&E (C) or Laminin (H) staining, respectively (scale bar 100 μm). Subsequently, measurements of adipocyte area (D, E) (n = 4) and myocyte (H, I) (n = 3 for PBS, n = 4 for H1573, n = 5 for H1573ΔGFP and H1573ΔGDF15) cross-sectional area were obtained. Gastrocnemius muscle was harvested and subjected to quantitative RT-PCR analysis of mouse mRNA levels of the indicated genes (n = 6 for non-tumor bearing mice group, n = 5 for tumor bearing mice group) (J). Data are shown as mean ± SEM of the actual measurements (A, B, D, F, G, and I), or as scattered plots with regression line and 95% confidence interval of relative values (J). P was calculated using 1-way (A, B, D–G, I) followed by Dunnett’s multiple-comparison test for significant differences from the H1573ΔGDF15 cohort. P was calculated using Pearson correlation coefficients, two-tailed (J). n.s. not significant. Source data are provided as a Source Data file.
