Table 2 Thermodynamic properties of non-canonical nucleic acids
From: Advances and challenges in non-canonical nucleic acids data storage
Type | ΔTm compared to DNA (°C) | ΔTm compared to RNA (°C) | Reference | |
|---|---|---|---|---|
Base modifications | 2-amino-A | +(2 ~ 4) | / | |
6mA | / | / | / | |
5mC | +(5 ~ 9) | / | ||
5hmC | / | / | / | |
8-oxoG | −(8.3 ~ 13.8) | / | ||
isoC-isoG | / | / | / | |
5-F-U | / | −0.8 | ||
5-Cl-U | / | +6.6 | ||
5-Br-U | / | +5.8 | ||
5-I-U | / | +4 | ||
5-Me-U | / | +0.6 | ||
5E-U | / | / | / | |
B-S, P-Z, X-K, J-V | / | / | / | |
Sugar modifications | 2′-OMe-RNA | +(0.7 ~ 1.1) | −0.6 | |
2F-RNA | / | +2.4 | ||
ANA | +(2 ~ 10.2) | +1.9 | ||
FANA | +(1 ~ 2) | / | ||
TNA | −(4.5 ~ 11) | / | ||
LNA | +(1 ~ 8) | +(2 ~ 10) | ||
L-αTNA | +4.1 | +0.3 | ||
D-αTNA | +4.2 | +3.0 | ||
XyloNA | / | / | / | |
Backbone modifications | PS-DNA | +(2.2 ~ 2.6) | −0.7 | |
PS-RNA | +2.4 | / | / | |
NP-DNA | −0.5 | −0.7 | ||
PNA | +(0.2 ~ 1.2) | +1.3 | ||
SNA | +(0.5-12) | / | ||
Multiple modifications | PS + FANA | / | / | / |
TNA + TPT3 | / | / | / | |
Mirror-image isomerism | L-DNA | / | / | / |
L-RNA | / | / | / | |
