Fig. 5: Biological context–dependent differences in cytosolic accumulation.
From: Click-based determination of accumulation of molecules in Escherichia coli
a Schematic representation of the scenario of a high accumulation molecule and a low accumulation molecule in intact WT E. coli relative to WT E. coli treated with PMBN. The disruption to the membrane barriers upon PMBN treatment should primarily alter the accumulation profile of molecules whose apparent accumulations are low in intact WT E. coli. b Comparison of apparent accumulation of 400+ small molecules in E. coli using CHAMP across two biological contexts: PMBN co-incubated WT rough E. coli and WT rough E. coli. c Analysis of the 8 different physicochemical parameters that can potentially impact accumulation profiles of the 400+ small molecules (HBA denotes hydrogen bond acceptor; HBD denotes hydrogen bond donor). For all CHAMP assays, E. coli cells carrying the HaloTag-expressing plasmid were induced with IPTG, treated with DBCOcl, then incubated with the azide-tagged test molecules for 1 h in PBS at 37 °C.
