Table 1 Bacterial strains and plasmids used in this study
From: Click-based determination of accumulation of molecules in Escherichia coli
Strain and Plasmid | Relevant characteristics | Source or reference |
|---|---|---|
E. coli BW25113 | WT K12 Keio parent strain; E. coli background | Horizon Discovery Ltd |
E. coli BW25113 attTn7::mini Tn7T Gmr fhuAC/4L | K12 Keio parent strain; constitutive expression of the FhuA pore (non-selective variant of FhuA); gentamicin 20 μg/ml) | Brown Lab, McMaster |
E. coli BW25113 ∆tolC::kanR | K12 ΔtolC, KanR (Keio collection, tolC deletion strain; kanamycin,50 μg/ml) | Horizon Discovery Ltd |
E. coli BW25113 ∆tolC::kanR attTn7::mini Tn7T Gmr fhuAC/4L70 | K12 ΔtolC, KanR; constitutive expression of the FhuA pore (non-selective variant of FhuA) GentR | Brown Lab, McMaster |
E. coli ATCC25922 | Wild type, smooth, O-antigen | ATCC |
pQE-30 | T5 promoter, AmpR | Genscript |
