Fig. 6: scCHyMErA-Seq enables high-throughput and accurate identification of exons and genes that modulate transcriptional phenotypes. | Nature Communications

Fig. 6: scCHyMErA-Seq enables high-throughput and accurate identification of exons and genes that modulate transcriptional phenotypes.

From: Single-cell exon deletion profiling reveals splicing events that shape gene expression and cell state dynamics

Fig. 6: scCHyMErA-Seq enables high-throughput and accurate identification of exons and genes that modulate transcriptional phenotypes.

a, c, e Venn diagrams showing the overlap of differentially expressed genes regulated by perturbation of NRF1 exon-7 (a, c) or TAF5 exon-8 (e). Overlaps are compared between our original scCHyMErA-Seq screen in HAP1 cells and either a small-scale replicate screen in HAP1 cells (a) or bulk RNA-Seq in HEK293 cells following isoform rescue experiments (c, e). Statistical significance was determined by two-sided Fisher’s exact test (****p < 0.0001), and the corresponding odds ratios (OR) are indicated. b, d, f Scatterplots showing the Pearson correlation of log2 fold-changes for differentially expressed genes identified by scCHyMErA-Seq and either a replicate screen (b) or bulk RNA-Seq for NRF1 exon-7 (d) and TAF5 exon-8 (f) perturbations. Each point represents a gene; Two-sided Pearson correlation coefficients with corresponding significance values are indicated.

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