Fig. 2: Substrate scope of the photoenzymatic enantioselective CN translocation reaction.
From: Stereoselective cyano translocation reaction enabled by photoenzymatic catalysis
The reaction conditions of 1a-1m: 1a-1m (3 mM), CsER (1 mol%), NADP+ (0.1 μmol), GDH (0.1 mg, about 3 U), glucose (100 μmol), solvent (960 μL, 50 mM tris buffer, pH 8.0 and 40 μL DMSO), at room temperature with the irradiation Blue LEDs reaction for 12 h under N2 atmosphere; The reaction conditions of 1a and 1f: 1a or 1f (3 mM), GluER (1 mol%), NADP+ (0.1 μmol), GDH (0.1 mg, about 3 U), glucose (100 μmol), solvent (960 μL, 50 mM tris buffer, pH 8.0 and 40 μL DMSO), at room temperature with the irradiation Blue LEDs reaction for 12 h under N2 atmosphere; The reaction conditions of 1n-1u: 1n-1u (3 mM), OYE3-Y196F (1 mol%), NADP+ (0.1 μmol), GDH (0.1 mg, about 3 U), glucose (100 μmol), solvent (960 μL, 50 mM KPi buffer, pH 8.0 and 40 μL CH3CN), at room temperature with the irradiation Blue LEDs reaction for 12 h under N2 atmosphere; a, Isolated yield based on a 0.1-mmol reaction. Yield were determined by HPLC and enantiomeric ratio (e.r.) were determined by GC or HPLC.
