Fig. 5: CMoRE recognizes 5hmC.
From: A bacterial defense system targeting modified cytosine of phage genomic DNA
a Crystal structures of CMoRE complexed with 5hm-dCTP. The electron density maps of 5hm-dCTP (Fo-Fc omit map, contoured at 3 σ) are shown. The details of the 5hmC binding are shown in the enlarged part, and the 5hmC recognition of the SRA domain of UHRF2 (PDB ID 4PW6) is shown for comparison. b Structural superposition reveals a conserved ligand-binding conformation. c Measured distances between 5hmC-binding sites across CMoRE tetramers. The CMoRE tetramers are shown in surface and the 5hmC molecules are shown in balls. d Proposed model for the anti-phage mechanism of the CMoRE system.
