Fig. 6: bioPROTAC-loaded hubs function as reaction centers for target degradation in cells.
From: Delivery of peptide coacervates to form stable interaction hubs in cells
A Scheme showing bioPROTAC-loaded HBpep-SA coacervates delivered to GFP expressing cells and subsequent degradation of target GFP. B Representative fluorescence images set to same black and white values showing GFP flurecence in monoclonal GFP expressing cell line after delivery of coacervate hubs (preformed HBpep-SA coacervates containing either no cargo or 1.35 µM of the following: VHHGFP4 SPOP167–374 or bioPROTAC VHHGFP4-SPOP167–374. Scale bar: 10 µm. C Quantification of normalized average cytosolic target GFP fluorescence intensity. n = 5 repeats. Error bars indicate standard deviation from the mean. Center of error bars indicate the mean. Ordinary one-way ANOVA tests were used. Šidák correction is used to correct for multiple comparisons. P-value is 0.9251 for ns, and P ≤ 0.0001 for other comparisons. Source data are provided as a Source Data file.
