Fig. 1: IMC-M113V is active against prevalent variants of Gag77–85.
a Schematic of IMC-M113V consisting of an affinity-enhanced TCR as the targeting domain and an anti-CD3 single-chain variable fragment (scFV) as the effector domain. b The binding affinity of IMC-M113V to the Gag77–85 cognate peptide sequence and the 8 most prevalent variants was determined by surface plasmon resonance (25 °C) and is presented as KD values. Stability of the Gag77–85 variants in complex with HLA-A*02:01 was determined from t1/2 at 37 °C. c Infection of HLA-A*02:01-transduced C8166 cells with HIV-1 IIIB was confirmed by intracellular HIV Gag p24 staining. d Confirmed uninfected or infected C8166 cells were stained with the rabbit Fc-tagged TCR domain of IMC-M113VRES and goat anti-rabbit CF640R. TCRs bound to peptide-HLA complexes on infected cells were quantified by total internal reflection microscopy. Each dot represents one cell. No TCR control represents infected cells stained with goat anti-rabbit CF640R only. Horizontal bars indicate median values, and indigo dotted lines indicate upper and lower quartiles. Statistical significance was determined using 1-way analysis of variance (ANOVA) with Tukey multiple comparisons test.
