Fig. 2: Molecular markers and a single-nuclei atlas for the proventriculus.

A 71F06-Gal4 drives specific expression in Zone 4 cells, including EdU+ cells. Representative image is shown from > 10 independent stainings. B UMAP projection of a single snRNAseq dataset created from 22,332 nuclei, containing 14 clusters representing the major epithelial, muscle, and neuronal cells found in the proventriculus. C Schematic of the major epithelial cell types in the proventriculus, corresponding to the clusters identified in (B). D Dot plot of selected marker genes for the 12 major clusters identified via snRNAseq; note that two minor clusters (“anterior midgut 1” and “unknown”) are excluded from this figure as they could not be clearly linked to in vivo cell types. E Expression patterns of marker genes for each of the 9 major epithelial cell types in the proventriculus. Anterior is up. Confocal z-slices are shown.