Fig. 5: Microglia ablation via PLX5622 does not lead to increased SGZ adult neurogenesis.
A Neural stem cell-specific mouse model genetically labeling adult-born neurons to examine the effects of microglial ablation on DCX+ cells (B, C). Representative images showing tdTomato, DCX, and IBA1 expression in B NestinCreER-Ai9 (tdTomato) reporter mice given a control diet or C PLX5622 diet. D–G Quantification for D the number of tdTomato+ cells E DCX+ cells F the colocalization of tdTomato + /DCX+ cells in the SGZ, and G the number of IBA1+ cells in control or PLX5622 treated mice. D (n = 4 for control and n = 6 for PLX, ns = not significant); E (n = 4 for control and n = 6 for PLX, ns = not significant); F (n = 4 for control and n = 6 for PLX, ns= not significant); G (n = 5 for control and n = 10 for PLX, ****p < 0.001). Each data point represents the average of a single animal (3–6 brain sections per mouse). The sex of each animal is represented by open circles (females) and open triangles (males). Mean ± SE. Two-sided Student’s t-test was used for all panels. Scale bar = 100 µm. Panel A was created in BioRender. Luo, A. (2026) https://BioRender.com/k88gw1g. Source data are provided as a source data file.
