Fig. 3: Enhanced systemic replication of Bovine and Human H5N1 viruses compared to the Mountain Lion H5N1 virus.
A Hamsters (n = 6 per group) were inoculated with Bovine, Mountain Lion, or Human H5N1 viruses, and tissues were collected at 4 dpi. Virus titers were quantified and are presented as log10 transformed values of TCID₅₀/g. Statistical significance was assessed using Two-way ANOVA followed by Tukey’s multiple comparisons test. P values adjusted for multiple comparisons and < 0.05 are indicated. Data are represented as box-and-whisker plots depicting the median (center line) and interquartile range (25th-75th percentiles). Whiskers indicate minimum and maximum values and all individual data points are shown. The limit of detection was 0.5 log10 TCID50/g. Bars represent the range (minimum to maximum), with the middle line indicating the median. B Influenza A NP antigen distribution was assessed by IHC. Representative IHC images of Nasal turbinates (NT), trachea, lung, spleen, liver, kidney, and brain are shown from two animals per group, one exhibiting systemic involvement and one without. Magnification and scale bars: 200×, bar = 50 µm.
