Fig. 3: Sucnr1 restricts HSPC and hematopoiesis under steady-state.

Analysis of hematopoiesis in C57BL6/J wild-type (WT) and Sucnr1 knock-out (Sucnr1-KO) mice (n = 10 WT, n = 6 Sucnr1-KO, 53-54 weeks old, unless indicated otherwise). A Numbers of white blood cells (WBC) measured with hematological analyzer and CD11b⁺ myeloid cells, CD11b⁺Gr-1^hiF4/80⁻ granulocytes, CD11b⁺Gr-1⁺F4/80⁺ monocytes, B220⁺ B lymphocytes and CD3⁺ T lymphocytes per ml of peripheral blood (PB) analyzed by fluorescence-activated cell sorting (FACS) (n = 11 WT, n = 12 Sucnr1-KO, 38-41 weeks old). B Total bone marrow (TBM) nucleated cells measured with cell counter. C CD11b⁺ myeloid cells, CD11b⁺Gr-1^hiF4/80⁻ granulocytes, CD11b⁺Gr-1⁺F4/80⁺ monocytes, B220⁺ B lymphocytes and CD3⁺ T lymphocytes analyzed by FACS. D TBM number of Lin⁻Sca-1⁺c-Kit⁺ (LSK) cells analyzed by FACS. E TBM numbers of LSK cell subsets: LSK CD34⁻Flt3⁻CD150⁺CD48⁻, hematopoietic stem cells (HSC); LSK CD34⁺Flt3⁻CD150⁺CD48⁻, multipotent progenitors 1 (MPP1); LSK CD34⁺Flt3⁻CD150⁺CD48⁺ (MPP2); LSK CD34⁺Flt3⁻CD150⁻CD48⁺ (MPP3); LSK CD34⁺Flt3⁺CD150⁻CD48⁺ (MPP4); LSK CD34⁺Flt3⁻CD150⁻CD48⁻ (MPP5); LSK CD34⁻Flt3⁻CD150⁻CD48⁻ (MPP6) analyzed by FACS. F TBM numbers of colony-forming unit cells (CFU-C) ex vivo after serial replating (n = 12 WT, n = 11 Sucnr1-KO 1–2 wk, n = 10 Sucnr1-KO 3 wk; wk, weeks). G TBM number of Lin⁻c-Kit⁺Sca-1⁻ (LK) progenitors analyzed by FACS. H TBM numbers of common lymphoid progenitors (CLP, Lin⁻c-Kit^lowSca-1^lowCD127⁺), common myeloid progenitors (CMP, LK CD34⁺FcRγ⁻), megakaryocyte erythroid progenitors (MEP, LK CD34⁻FcRγ⁻) and granulocyte-monocyte progenitors (GMP, LK CD34⁺FcRγ⁺) analyzed by FACS. Statistical analyses were performed with two-tailed Student’s t test (A–D, E except MPP1 and MPP5, F, G, H except CLP) or two-tailed Mann–Whitney U test (E MPP1 and MPP5, H CLP). Significant p-values are reported. Data are biologically independent animals, and means ± SEM. Source data and non-significant p-values are provided as a Source Data file.