Fig. 4: Sucnr1 restricts HSPC and hematopoiesis cell-autonomously.
From: Succinate receptor 1 restricts hematopoiesis and prevents acute myeloid leukemia progression
Transplant of C57BL6/J wild-type (WT) mice with bone marrow nucleated cells (BMNC) from Sucnr1fl/fl or Mx1-Cre Sucnr1fl/fl and induction 4 weeks after with poly-inosine:poly-cytosine (pIpC). A Illustration of the experimental design. R: recipient. D: donor cells. The mouse, cell and beam icons: Created in BioRender. Cuminetti, V. (2026) https://BioRender.com/9bm56nx. B Evolution in number of white blood cells (WBC) per ml of peripheral blood (PB) measured with hematological analyzer (n = 8 Sucnr1fl/fl, n = 8 weeks 0–4 and n = 7 weeks 8–16 Mx1-Cre Sucnr1fl/fl), and in numbers of CD11b+ myeloid cells (n = 8 weeks 0–4, n = 7 weeks 8, 16 and n = 6 week 12 Sucnr1fl/fl; n = 8 weeks 0–4, n = 7 week 8 and n = 6 weeks 12–16 Mx1-Cre Sucnr1fl/fl), CD11b+Gr-1+F4/80+ monocytes (n = 8 weeks 0–12 and n = 7 week 16 Sucnr1fl/fl, n = 8 weeks 0–4, n = 7 week 8 and n = 6 weeks 12–16 Mx1-Cre Sucnr1fl/fl) and B220+ B lymphocytes (n = 8 weeks 0–4, 12–16 and n = 7 week 8 Sucnr1fl/fl, n = 8 weeks 0–4, n = 7 weeks 8, 16 and n = 6 week 12 Mx1-Cre Sucnr1fl/fl) per ml of PB analyzed by fluorescence-activated cell sorting (FACS). C Number of WBC (n = 8 Sucnr1fl/fl, n = 7 Mx1-Cre Sucnr1fl/fl), and numbers of CD11b+Gr-1+F4/80+ monocytes (n = 7 per group) and B220+ B lymphocytes (n = 8 Sucnr1fl/fl, n = 7 Mx1-Cre Sucnr1fl/fl) per ml of PB, 47 weeks after pIpC. D (Left) Fractions of Lin−Sca-1+c-Kit+ (LSK) subsets; long-term hematopoietic stem cells (LT, LSK CD34−Flt3−), short-term hematopoietic stem cells (ST, LSK CD34+Flt3−) and multipotent progenitors (MPP, LSK CD34+Flt3+) within the LSK cell compartment analyzed by FACS (n = 7 Sucnr1fl/fl, n = 6 Mx1-Cre Sucnr1fl/fl). (Right) Total BM (TBM) numbers of LSK cell subsets: LSK CD34−Flt3−CD150+CD48−, hematopoietic stem cells (HSC); LSK CD34+Flt3−CD150+CD48−, multipotent progenitors 1 (MPP1); LSK CD34+Flt3−CD150+CD48+ (MPP2); LSK CD34+Flt3−CD150−CD48+ (MPP3); LSK CD34+Flt3+CD150−CD48+ (MPP4); LSK CD34+Flt3−CD150−CD48− (MPP5); LSK CD34−Flt3−CD150−CD48− (MPP6) analyzed by FACS (n = 7 all except n = 6 MPP3 Sucnr1fl/fl, n = 5 HSC, MPP1-3 and n = 6 MPP4-6 Mx1-Cre Sucnr1fl/fl). Statistical analyses were performed with two-tailed Student’s t-test (B, C, D except MPP4) or two-tailed Mann–Whitney U test (D MPP4). Significant p-values are reported. Data are biologically independent animals, and means ± SEM for bar plots. Data are mean ± SEM in (B) for better visualization. Source data and non-significant p-values are provided as a Source Data file.
