Fig. 6: State specific intrabody interactions with SOD1 and α-synuclein.
From: Reliable repurposing of the antibody interactome inside the cell
a Assessment of the interaction between scFv intrabodies derived from anti-SOD1 conformation specific antibodies with wild-type, A4V, G93A and D90A SOD1 by co-immunoprecipitation on anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated on the left and right. b SOD1 conformation specific intrabodies target five distinct epitopes in the SOD1 monomer here shown in the fully metalated disulphide intact form. c Immunoprecipitation of A4V SOD1 by MS785-derived scFv but not Z-FUS-derived scFv. d Oligomeric state specificity of five anti-α-synuclein antibodies. e Co-immunoprecipitation of α-synuclein by scFv intrabodies derived from antibodies in (d) on anti-HA beads and visualised by SDS-PAGE/western blot. Antibodies used to probe western membranes are stated. IP Immunoprecipitation.
