Table 6 Staining procedures

Halo PA-JF646

75 nM, overnight

Halo^ER

Fig. 2g

Halo PA-JF646

25 nM, overnight

Halo^cyto

Fig. 3f

Halo PA-JF646

0.5 µM, 1 min

Halo^Mito

Fig. 4a

Halo PA-JF646

0.5 µM, 1 min

TOMM20::Halo

Fig. 4a

Halo PA-JF646

0.5 µM, 1 min

SEC61B::Halo

Fig. 4a

cpSNAP JF549

0.25 μM, 15 min

SEC13::Snap

Fig. 5a

Halo PA-JF646

1 μM, 15 min

Halo^ER

Fig. 5b

Halo PA-JF646

5 μM, 30 min

APP::Halo

Fig. 6b

Halo PA-JF646

75 nM, overnight

intrabody^ER::Halo

Fig. 7c

Snap PA-JF646

1.5 μM, 1 h

APP::Snap

Fig. 7c