Table 7 Tracking parameters for experimental data

From: FidlTrack: high-fidelity structure-aware single particle tracking resolves intracellular molecular motion in organelles sensing APP processing

Dataset

Figures

Framerate (Hz)

Spot radius (μm)

Spot threshold

Max. linking distance (μm)

Min. traj. length

HaloER

Fig. 2g-k

50

0.55

0.3

2.8 (conventional)

15

HaloCyto

Fig. 3f-i

41.7

0.75

0.2

6 (conventional & struct-aware)

5

HaloMito

Fig. 4a-f

167

0.75

1.1

1 (struct-aware)

10

TOMM20

Fig. 4a-f

167

0.75

1

0.6 (struct-aware)

10

SEC61B

Fig. 4a-f

167

0.75

1

0.6 (struct-aware)

5

SEC61B (BSA / OA)

Fig. 4g,h

167

0.75

1

1 (struct-aware)

5

HaloER

Fig. 5

100

0.75

6

2 (conventional & struct-aware)

10

APP (N-term)

Fig. 6

100

0.6

0.5

1 (conventional & struct-aware)

20

AlfatagER

Fig. 7

167

0.7

0.35

1 (struct-aware)

5

APP

Fig. 7

167

0.7

0.35

1 (struct-aware)

5

  1. Spot radii and detection threshold are used in the spot detection process, the maximum linking distance is used in the tracking and the minimum trajectory length is a post-processing step.