Fig. 3: Identification of the natural redox partners of DoxA. | Nature Communications

Fig. 3: Identification of the natural redox partners of DoxA.

From: Metabolic engineering of doxorubicin biosynthesis through P450-redox partner optimization and structural analysis of DoxA

Fig. 3: Identification of the natural redox partners of DoxA.

A DoxA reactions with non-native redox partners PDX + PDR from P. putida and SFX + SFR from spinach using DOD as a substrate, B Phylogenetic tree of putative FDR enzymes, including putidaredoxin-type (purple), adrenodoxin-type (blue), and plant-type ferredoxin reductases (pink) identified from the genome of S. peucetius ATCC27952. Reference sequences for putidaredoxin reductase-type (BphA4, Pseudomonas sp.; PdR, Pseudomonas putida; SmFdR, Stenotrophomonas maltophilia), adrenoredoxin reductase-type (AtFdR, Arabidopsis thaliana; MtFdR, Mycobacterium tuberculosis), and plant ferredoxin reductase-type (ZmFdR, Zea mays; SoFdR, Spinacia oleracea; PsFdR, Pisum sativum) were used to guide the tree. Proteins marked with an asterisk (*) could be produced in soluble form and were used in enzyme activity assays, C DoxA reactions with FDX4 and various ferredoxin reductase partners using DOD as a substrate.

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