Fig. 4: High-quality electrostatic potential and electron density maps obtained from micro- and nano-volume 3D electron diffraction (ED) and serial synchrotron-radiation X-ray crystallography (SSX) data collected from in cellulo MgHEX-1 crystals.

Electrostatic scattering potential maps 2Fo-Fc contoured at 1σ (grey) calculated from the micro- (a) and nano- (b) volume 3D ED data. The modelled MgHEX-1 protein structures are shown in green (N-terminal domain) and blue (C-terminal domain) stick representation. For direct comparison, the electron density map obtained from serial X-ray diffraction of approximately 62,500 intracellular MgHEX-1 crystals is presented (c). Selected regions (1, residues Thr65 to His72 and Ser88 to Gln101; 2, residues Gly105 to Lys109) of the electrostatic potential and electron density maps (red squares) are shown in detail for the micro- (d) and the nano- (e) volume 3D ED data as well as for the SSX data (f).