Fig. 4: Loss of FMRP in cortical excitatory neurons and glia is sufficient for the alpha phenotype in L4 of V1.

a Inset: Experimental design. V1 L4 LFP data were collected from juvenile (p30–40) mice with Fmr1 specifically knocked out of cortical excitatory neurons and glia expressing the Emx1 promotor (Emx1-Fmr1 KO mice; Cre+/Fmr1-, n = 15) and WT littermates (Cre/WT mice; Cre+/Fmr1+, n = 10 and WT/KO mice; Cre-/Fmr1-, n = 10). Mice were head-fixed in front of a monitor displaying a static, iso-luminant gray screen and habituated to head-fixation for two days before data collection. Main: Absolute power spectrum (mean +/− SEM). b Main: Periodic power spectrum (mean +/− SEM). Inset: Peak 1 (Pk1) at a higher spectral resolution. c−f Boxplot and FDR-corrected p-values for: c Pk1a center frequency. Uncorrected p-value = 0.0229, z-statistic = −2.275, effect size = −0.457; d Pk1a maximum power. Uncorrected p-value = 5.606e−4, z-statistic = −3.45, effect size = −0.693; e Pk1b center frequency. Z-statistic = −0.1, effect size = −0.023; and f Pk1b maximum power. Uncorrected p-value = 2.984e−4, z-statistic = −3.617, effect size = −0.727. Dots at the bottom of plots in (a, b) indicate the points of significant difference between groups (non-parametric hierarchical bootstrap, 99% confidence interval). Boxplots show 25th, median, and 75th percentiles, with whiskers extending to minimum and maximum values. Uncorrected p-values and z-statistics calculated from two-sample, two-sided Wilcoxon Rank-Sum Tests and effect sizes calculated using Cliff’s Delta. P-values adjusted with the Benjamini-Hochberg correction. Source data are provided as a Source Data file.