Fig. 5: Small molecule-mediated enhancement of condensate viscoelasticity.
From: Decoupling phase separation and fibrillization preserves activity of biomolecular condensates
a Schematic of video particle-tracking (VPT) nanorheology measurements using 200 nm probe particles passively embedded within Tau condensates. b Representative mean squared displacement (MSD) measurements of probe particles inside Tau condensates either with or without small molecule treatment (2 mM L-Arg). The measurements were conducted 2 h after sample preparation. c Representative dynamical moduli of Tau condensates either in the absence (left) or presence (right) of 2 mM L-Arg. The measurements were conducted 2 h after sample preparation. The dashed line represents the crossover frequency. d Reports a diagram-of-states for untreated (UT) and small molecule-treated (L-Arg) Tau condensates at the indicated sample age, based on the estimated moduli at 0.15 Hz. The experimentally determined data are represented as mean values with standard deviations for n = 3 independent replicates. The sample size for all these measurements is 3 condensates from three independent replicates. Source data are provided as a Source Data file.
