Fig. 2: Gpr126-NTF and -CTF are required for trabeculation. | Nature Communications

Fig. 2: Gpr126-NTF and -CTF are required for trabeculation.

From: Adgrg6/Gpr126 is required for compact wall integrity and establishing trabecular identity during cardiac trabeculation

Fig. 2: Gpr126-NTF and -CTF are required for trabeculation.

a Representative confocal images (mid-sagittal sections) of hearts of WT, zygotic gpr126stl47, and zygotic gpr126st49 larvae crossed into Tg(myl7:EGFP-hsa.HRAS) background at 120 h post fertilization (hpf). Arrowheads: trabeculae, Arrows: multilayered ventricular wall. V: ventricle. At: atrium. Scale bars: 20 µm. b Quantification of trabeculation phenotypes in 120 hpf WT (n = 11), zygotic gpr126stl47 (n = 12), and zygotic gpr126st49 (n = 15) larvae. c Representative confocal images (mid-sagittal sections) of hearts of WT, maternal zygotic (MZ) gpr126stl47, and MZ gpr126st49 larvae crossed into Tg(myl7:EGFP-hsa.HRAS) background at 96 and 120 hpf. Yellow arrowheads: trabeculae, orange arrowheads: shorter trabeculae Asterisks: absence of trabeculae. Arrows: multilayered ventricular wall. V: ventricle. At: atrium. Scale bars: 20 µm. d Quantification of trabeculation phenotypes in 120 hpf WT (n = 20), MZ gpr126stl47 (n = 30), and MZ gpr126st49 (n = 30) larvae. e 3D surface rendering of the myocardium of 120 hpf WT and mutants as indicated. Arrowheads: trabecular projections. Scale bars: 20 µm.

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