Fig. 3: Endogenous NO production from nNOSOV/MePO neurons triggers a surge-like LH release pattern. | Nature Communications

Fig. 3: Endogenous NO production from nNOSOV/MePO neurons triggers a surge-like LH release pattern.

From: Preoptic kisspeptin-nNOS-GnRH (KiNG) neuronal network regulates LH rhythmicity through activation-inhibition in mice

Fig. 3: Endogenous NO production from nNOSOV/MePO neurons triggers a surge-like LH release pattern.

A Graphical illustration of the strategy for the selective expression of AAV9-hM3D(Gq)-mCherry targeted to nNOSOV/MePO neurons in Nos1cre female mice. B Confocal images show RFP, nNOS and colocalization immunoreactivity in the OV/MePO. C Graph of the LH release pattern over 180 min of serial blood sampling after administration of saline or L-NAME, 30 min before the beginning of the sampling. Arrow indicates the time of CNO administration (65th minute) (two-way ANOVA, Sidak’s multiple comparisons test, time x treatment, F (11, 110) = 4.23 p < 0.001; minute 160 p = 0.03, minute 170 p = 0.009, minute 180 p = 0.008; n = 6). Quantification of the D area under the curve (two-way ANOVA, Uncorrected Fisher’s LSD, CNO effect: F(1, 10) = 24.18 p < 0.001; saline pre CNO vs post CNO, p < 0.001; treatment effect: F(1, 10) = 4.48 p = 0.06 post CNO saline vs L-NAME, p = 0.008; n = 6), E mean LH (two-way ANOVA, Uncorrected Fisher’s LSD; CNO effect: F(1, 10) = 25.38 p < 0.001; saline pre CNO vs post CNO, p < 0.001; treatment effect: F(1, 10) = 4.33 p = 0.06; post CNO saline vs L-NAME, p = 0.007; n = 6), F basal LH (two-way ANOVA, Uncorrected Fisher’s LSD, CNO effect: F(1, 10) = 25.84 p < 0.001; saline pre CNO vs post CNO, p = 0.003; L-NAME pre CNO vs post CNO, p = 0.008; n = 6), and G peak LH (two-way ANOVA, Uncorrected Fisher’s LSD, CNO effect: F(1, 10) = 16.45 p = 0.002; saline pre CNO vs post CNO, p = 0.001; treatment effect: F(1, 10) = 3.87 p = 0.002; post CNO saline vs L-NAME, p = 0.007; n = 6) levels of the Nos1cre animals before and after CNO administration in saline and L-NAME injected animals. Experiment was independently repeated twice with similar results. Values indicate means ± SEM. ns: non-significant, p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file. Schematic illustrations created in BioRender. Chachlaki, K. (2026) https://BioRender.com/arnv9ca. 3 V, third ventricle. OV, organum vasculosum laminae terminalis. MePO, median preoptic nucleus. kp-10, kisspeptin-10. NO, nitric oxide. CNO, Clozapine N-oxide. AUC, area under the curve. LH, luteinizing hormone.

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