Fig. 4: Cryo-EM data processing and statistics of control fibrils vs annealed fibrils.
From: Structural defects in amyloid-β fibrils drive secondary nucleation
a, b Motion corrected micrographs showing control fibrils and annealed fibrils, respectively. c, d 2D class averages for twisted fibrils in control fibrils and annealed fibrils datasets, respectively, and measurements of fibril diameter at points along the fibril crossover. e, f 2D class averages for untwisted fibrils in 37 °C fibrils and annealed fibrils datasets, respectively. g summary of fibril morph population and morphology statistics. Cryo-TEM images of Aβ40 (h) or Aβ42 (i, j and k) fibrils showing evidence of growth defects, as pointed by the white arrows. The scale bar shown as white line is 50 nm. The Aβ40 fibrils in (h) are formed in a solution of 10 μM Aβ40 at pH 7.4, 37 °C, while Aβ42 fibrils in (i, j and k) are formed in a solution of 5 μM Aβ42 at pH 8, 37 °C. White arrows point to the locations of the defects. Further details of the cryo-EM processing workflow are found in Supplementary Fig. 7.
