Fig. 3: PACT monitoring of CSF drainage in AD and WT mice.
From: Photoacoustic computed tomography monitors cerebrospinal fluid dynamics and glymphatic function
PACT whole-body MAP images in the XY and YZ planes of a WT mice and b AD model mice. Images were captured at pre-injection and 1, 6, 24, and 96 hours after injection of ICG. The orange dashed boxes indicate the zoomed-in brain region in the YZ-MAP images, and the orange arrows indicate the injection site in the striatum. c Changes over time in the ICG striatum signals in WT mice and AD mice in the ICG unmixed map (WT: n = 8, AD: n = 8). d Ex vivo FL brain images acquired using the IVIS system at 96 h after injection of ICG. e Comparison of the ex vivo FL intensities of the striatum between WT mice and AD mice at 96 hours after injection of ICG (WT: n = 3, AD: n = 3). Comparisons were performed using an independent two-tailed t-test t(4) = −4.65, p = 0.0096, d = 5.77, 95% CI = [−9.5 × 108 −4.9×108] *p < 0.05; **p < 0.01; ***p < 0.005; CSF, cerebrospinal fluid; PACT, photoacoustic computed tomography; PA, photoacoustic; MAP, maximum amplitude projection; ICG, indocyanine green; AD, Alzheimer’s disease; WT, wild type; FL, fluorescence; arb. units, arbitrary units. All statistical data are presented as mean ± standard errors, and individual data are overlaid as dot plots.
