Fig. 3: Chromatin accessibility patterns in normal hematopoietic cells at lineage-enriched perturbed ESLs.
From: Blood-based epigenetic instability linked to human aging and disease
a, b UMAP projections of single-cell ATAC-seq profiles from normal blood cells (n = 22,737). Cells are colored by their relative chromatin accessibility at genomic regions overlapping a lymphoid-enriched or b myeloid-enriched ESLs. Accessibility was quantified using deviation scores that reflect enrichment relative to a background model. Red indicates higher relative accessibility (less compacted chromatin), and blue indicates lower relative accessibility (more compacted chromatin). c, d Relative chromatin accessibility at ESL-associated regions in hematopoietic stem and progenitor cells (HSCs, CMPs, GMPs; n = 8321) compared with lymphoid cell types (CLP, Pre-B, B, CD4⁺ T, CD8⁺ T, NK; n = 14,416), for c lymphoid-enriched ESLs and d myeloid-enriched ESLs. For boxplots: black line represents the median, bounds of the box represent the first and third quartiles, whiskers extend to (median ± 1.5 * interquartile range), and points represent outliers. ****P < 2.2 × 10−16 by two-sided Wilcoxon rank-sum test. ATAC-seq assay for transposase-accessible chromatin using sequencing; UMAP uniform manifold approximation and projection, HSC hematopoietic stem cell, CMP common myeloid progenitor, GMP granulocyte-monocyte progenitor, CLP common lymphoid progenitor, NK natural killer. Source data are provided as a Source Data file.
