Fig. 5: Amulet neurons are postsynaptic to SELKs, and their activation promotes feeding.
From: Feeding decision-making by a single neuron via disparate neurotransmitters
a Rendering of the Amulet neurons from the EM reconstruction of a fly brain. Brain mesh is previously described46 and is under CC BY-NC 4.0 license (https://creativecommons.org/licenses/by-nc/4.0/). b The split Gal4 driver SS32423 provides genetic access to the Amulet neurons. Flies bearing the SS32423 driver and UAS-GFP are stained with an antibody against GFP (green). Neuropil is shown in blue. Scale bar: 50 μm. c The presynaptic inputs into the Amulet neurons are revealed by retro-Tango. The SS32423 driver drives retro-Tango from the Amulet neurons, revealing their presynaptic partners (magenta). SELKs are marked by staining with an antibody against LK (green). The merge image shows that SELKs (arrows) are costained with the presynaptic marker. Neuropil is shown in blue. Scale bar: 10 μm. d In a two-choice optoPAD assay, the flies express CsChrimson in Amulet neurons using the SS32423 driver. The total number of sips taken from either sweet (magenta) or sweet paired with a pulse of light (cyan) are plotted. Genetic controls are presented in lighter shades. Paired t-test. Two-tailed P values are 0.030, 0.798, 0.928, and 0.018, respectively. Boxplots indicate median (middle line), 25th and 75th percentile (box), bars represent maximum and minimum. For all panels, n indicates biological replicates. Source data are provided as a Source data file.
