Fig. 6: Modeling variation of transgene expression in 4-week-old N. benthamiana plants.
From: Causes and consequences of experimental variation in Nicotiana benthamiana transient expression
A Per-plant CV of unnormalized GFP calculated from data compiled across many years and independent experiments. CV for a plant is calculated using all disks derived from that plant, regardless of the leaf. For EHA105, n = 726, and for GV3101, n = 1087. Violins show the distribution of all data. Boxes show the median and IQR, and whiskers show the minima and maxima, excluding outliers (beyond 1.5 IQR). B CDF of per-plant, unnormalized eGFP CVs. Solid gray, empirical data from 32 batches of at least 30 plants. Dotted black, Monte Carlo simulated data. C Minimum number of simulated plants needed to detect a given effect size with the CV of unnormalized EHA105, unnormalized GV3101, or optimally normalized GV3101, assuming 95% accuracy and statistical significance (p < 0.05) for a two-tailed Student’s t-test. D Exponential regression fit to the Monte Carlo simulated data in (C), which are shown as points. Dashed horizontal line indicates the arbitrary 50 plant cap, and dotted vertical lines indicate the smallest detectable effect size with 50 plants. Orange, EHA105. Gray, unnormalized GV3101. Teal, optimally normalized GV3101. Source data for this figure is available in the Source Data file.
